Quantitative and Specific Assay of14C-Labeled Staphylococcal Enterotoxin B

Rapid quantitative serological assay of staphylococcal enterotoxin B.

A simple, rapid method, based on the Oudin single diffusion technique, is described for the quantitative assay of staphylococcal enterotoxin B. The method yields reproducible results without close control of such assay variables as temperature, antiserum dilution, and assay time, provided that the ionic strength is maintained above 0.2 n sodium chloride equivalent.

Microtiter hemagglutination-inhibition assay for staphylococcal enterotoxin B.

The rapid microtiter technique was investigated as a means of facilitating the detection of staphylococcal enterotoxin B. With this technique, many samples were assayed simultaneously, and readable results were obtained in 3 hr. Other advantages of this method, in addition to speed, were the small quantity of reactants used, ease of reading, and reproducibility.

Homogeneous enzyme immune assay for staphylococcal enterotoxin B.

A simple homogeneous enzyme immune assay was developed for detection of staphylococcal enterotoxin B by a conjugate of beta-amylase coupled with the enterotoxin.

Staphylococcal Enterotoxin B and Related Pyrogenic Toxins

Staphylococcal Enterotoxin B and Related Toxins

312 Medical Aspects of Biological Warfare